Mario, Must I remind you of your heritage? Anyone coming through the Herzenberg lab must have been at one time a B cell person. As for the uselessness of T cells, their only job is to listen to what the B cell tells them to do (okay, so they listen to dendritic cells as well). As for "assuming" that the colors were to be used for phenotyping, I never mentioned phenotyping. I was very careful to stick to the precise term of "fluorochrome" as requested by Michal in the original e-mail (I didn't even use the terms FL1, 2,..). The instrument in question has some functionality, as I have stated, but the inability to use the "UV" for "fluorochromes" is the major issue. Randy T. Fischer NIH/NIAMS Building 10, Room 6D57 9000 Rockville Pike Bethesda, MD 20892 (301) 594-3537 fischer1@mail.nih.gov > ---------- > From: Mario Roederer > Sent: Thursday, October 31, 2002 1:59 PM > To: Fischer, Randy (NIH/NIAMS); Cytometry Mailing List > Subject: RE: Fluorochromes choice > > Oh, you B-cell immunologists. You assume that all colors are used > for immunophenotyping. > > That's like our current philosophy, that B cells are good for only > one thing: making reagents with which we can distinguish important T > cell subsets! > > mr > > > >Hi Michal, > > > >You are just another in the line of researchers who has an instrument > which > >has used a misleading terminology in its description. It does have three > >lasers, and two are fairly useful although there is no compensation > >circuitry between the 488 and 633 lasers (problem for APC and PE-Cy5 > >channels). The third laser as mentioned above is a "UV", but the problem > is > >that the "UV" laser is too far down in the "UV" spectrum to be beneficial > >for the "UV" excitable fluorochromes. So, unfortunately, this laser > >configuration is totally useless when trying to use the currently > available > >fluorochromes to do 6-color staining. > > > >Randy T. Fischer > >NIH/NIAMS > >Building 10, Room 6D57 > >9000 Rockville Pike > >Bethesda, MD 20892 > >(301) 594-3537 > >fischer1@mail.nih.gov > >
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