> Dear Joanne- > quick width does improve sorts on the Vantage SE. I employed it routinely > with high speed sorts say 15,000 per second 4 colours. in general also used > it for positive cell selection sorts ie gfp or single colour where the cells > are annoying to work with so itıs a great tool. You donıt have QW with > FACSDiVa as dead time doesnıt exist. you can use a width parameter on any > channel but I havenıt found it necessary. > there you go > hope this helps > gisele > > Hi All! > > Is anyone using Quick Width as a Pulse Processing parameter for high speed > sorting? I would be curious to know if you find it has improved the purity of > your sorts. I am particularly interested in its utility in situations where > you have cells that tend to stick together and you may get a positive cell and > a negative cell stuck together when transversing the laser beam and it gets > sorted together as a positive but when reanalyzed they are now separate cells > which appears as a contaminate. I think this tends to be more of a problem > when you are sorting based on one fluorescence parameter such as GFP. I would > appreciate any opinions and experiences from those that have used this > feature. > > Thanks as always-Joanne > > > > Joanne Lannigan, MS > > Director, Flow Cytometry Core Facility > > Jordan Hall, Room 7067 > > P.O. Box 800734 > > Charlottesville, VA 22908-0734 > > Office: 434-924-0274 > > Lab: 434-243-2695 > > Fax: 434-982-1071 > > email: joannelannigan@virginia.edu > > > > -- Gisele Knowles >>Director Centre for Cytometry and Scanning Microscopy Sunnybrook and Women's Research Institute Room B 210 2075 Bayview Avenue Toronto, ON 416.480.6100x7282 (office) x7284/5 (lab)
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