Hi, Please post responses to the list, and I'd be interested in hearing more generally about what non-radioactive assays are used in current practise. This issue has been around awhile now, LDL assay (Promega ?) seems to have both proponents & detractors.While several assays have been validated against chromium release assays, which remain the gold standard, somehow non-radioactive assays have not replaced routine use of the chromium release assay. We still use it to measure cytotoxicity. Is it a standardisation issue? Stan. > From: "Lam, Lee" <lxl7@CDC.GOV> > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > Subject: NK cytotoxicity assay with human whole blood > Date: Tue, 25 Jun 2002 11:49:56 -0400 > > Dear all, > Our lab would like to use flow cytometry for the measuring natural killer > cell cytotoxicity. Does anyone have a working protocol that can share with > us? Also, could we use 24 hrs. old human whole blood instead of PBMC for the > assay? It will be really appreciated if you can provide us with valuable > pointers. Thanks in advance. > Lee Lam > CDC > Atlanta, Georgia > Phone: 404-639-2725 > Fax: 404-639-4838 > E-mail: LXL7@CDC.GOV <mailto:LXL7@CDC.GOV> > > > >
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