Hi John, My guess it's a hairy cell leukemia! Check CD103 and CD25 and check TRAP . These cells, due to their "hairy" protrusions show up in the "monocyte" scatter window. Let me know what your final conclusion is. best regards, Dirk Prof. Dirk Van Bockstaele, PhD Antwerp University Hospital Laboratory of Hematology Head of Flow Cytometry & Molecular Diagnostics Wilrijkstraat 10 B-2650 Edegem Belgium. phone: 32 3 821 3900, fax 32 3 825 1148 e-mail: dirk.van.bockstaele@uza.be > ---------- > Van: jmgonzal@javeriana.edu.co[SMTP:jmgonzal@javeriana.edu.co] > Verzonden: donderdag 3 oktober 2002 22:53 > Aan: Cytometry Mailing List > Onderwerp: Lymphoma case??? > > <<Bestand: Lymhoma case.jpg>> > Dear Clinical Flowers: > > We have a case of a male patient 50 years old with pancytopenia, > splenomegaly and augmentation of retroperitoneal ganglia. > > We received BM sample and made some markers (see annex)with a non commun > location in the CD45+ gating estrategy of a group of cells CD10+, CD19+, > CD20+, CD22+ and HLA-DR+. These cells were large cells (FSC) and > initially pathologist believed in myeloid derived cells but you will see > markers. > > What other markers will be useful? Could be a lymphoma (origen)? > > JOHN MARIO GONZALEZ M.D., Ph.D. > Facultad de Ciencias > Pontificia Universidad Javeriana > Fax 00 57 1 2850503 > Phone 00 57 1 3208320 Ext 4085 >
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