RE: was Human IgE, now "why Basotest?"I think there is at little magic in
the Basotest. The 'activation buffer' contains some IL-3 to preactivate the
basophils without upregulation of CD63.
So indeed, Golgi-plug, activation buffer,... why not call the things by
there name and mention all the substances ?
(my € 0.02 )
Chris Bridts
Immunology
University of Antwerp
Belgium
-----Oorspronkelijk bericht-----
Van: Calman Prussin [mailto:CPRUSSIN@niaid.nih.gov]
Verzonden: maandag 8 april 2002 06:41
Aan: Cytometry Mailing List
Onderwerp: RE: was Human IgE, now "why Basotest?"
You can also just buy some CD63 mAb and some f-Met-Leu-Phe (positive
control). There is no magic to the "Basotest", as it is based on surface
detection of translocated/exocytosed CD63 in anti-IgE gated basophils.
Depending on how much anti-IgE you use for your activation, you may find
that you are no longer able to detect basophils with flurochrome labeled
anti-IgE. If that is the case, CD123 (IL-3R alpha)
works just about as well to identify basophils (gate on CD123high cells).
I guess I have pet peeve about trade names. Somehow I can't take it
seriously when people mention they used "Golgi-plug" rather than just say
"Brefeldin A"....or is that "Golgi-stop"???
Just my $ 0.02,
Calman
----------
From: Gerhard Nebe-von-Caron
Sent: Friday, April 5, 2002 11:13 AM
To: Cytometry Mailing List
Subject: RE: Human IgE
<<File: ATT01064.ATT>>
You might want to try the Basotest (http://www.orpegen.de/diagnos0.htm)
It
probably does what you want and includes the relevant control material.
Good luck
Gerhard Nebe-von-Caron
Research Scientist
SEAC - Safety and Environmental Assurance Centre
Applied Science & Technology Group
Unilever Colworth, Sharnbrook, Bedfordshire, MK44 1LQ, UK
Tel: +44 (0)1234 264822, Fax: +44 (0)1234 222552
E- mailto:Gerhard.Nebe-von-Caron@unilever.com
-----Original Message-----
From: Alfredo Vannacci [SMTP:vannacci@pharm.unifi.it]
Sent: Wednesday, April 03, 2002 1:21 PM
To: Cytometry Mailing List
Subject: Human IgE
Dear flowers,
I was wondering if anyone of you knows a company from which I could buy
policlonal human IgE.
I need them because we are studying human basophil activation by flow
cytometry and we wish to standardise their response to anti-human IgE by
saturating cell surface FcE-RI receptors.
Thank you in advance for your help.
Dr Alfredo Vannacci, MD
University of Florence
Department of Preclinical and Clinical Pharmacology
email: vannacci@pharm.unifi.it tel: +39(0)554271234
fax: +39(0)554271280
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