You can also just buy some CD63 mAb and some f-Met-Leu-Phe (positive control). There is no magic to the "Basotest", as it is based on surface detection of translocated/exocytosed CD63 in anti-IgE gated basophils. Depending on how much anti-IgE you use for your activation, you may find that you are no longer able to detect basophils with flurochrome labeled anti-IgE. If that is the case, CD123 (IL-3R alpha) works just about as well to identify basophils (gate on CD123high cells). I guess I have pet peeve about trade names. Somehow I can't take it seriously when people mention they used "Golgi-plug" rather than just say "Brefeldin A"....or is that "Golgi-stop"??? Just my $ 0.02, Calman > ---------- > From: Gerhard Nebe-von-Caron > Sent: Friday, April 5, 2002 11:13 AM > To: Cytometry Mailing List > Subject: RE: Human IgE > > <<File: ATT01064.ATT>> > You might want to try the Basotest (http://www.orpegen.de/diagnos0.htm) > It > probably does what you want and includes the relevant control material. > > Good luck > > Gerhard Nebe-von-Caron > > Research Scientist > SEAC - Safety and Environmental Assurance Centre > Applied Science & Technology Group > Unilever Colworth, Sharnbrook, Bedfordshire, MK44 1LQ, UK > > Tel: +44 (0)1234 264822, Fax: +44 (0)1234 222552 > E- mailto:Gerhard.Nebe-von-Caron@unilever.com > > > > -----Original Message----- > From: Alfredo Vannacci [SMTP:vannacci@pharm.unifi.it] > Sent: Wednesday, April 03, 2002 1:21 PM > To: Cytometry Mailing List > Subject: Human IgE > > > Dear flowers, > I was wondering if anyone of you knows a company from which I could buy > policlonal human IgE. > I need them because we are studying human basophil activation by flow > cytometry and we wish to standardise their response to anti-human IgE by > saturating cell surface FcE-RI receptors. > Thank you in advance for your help. > > Dr Alfredo Vannacci, MD > University of Florence > Department of Preclinical and Clinical Pharmacology > email: vannacci@pharm.unifi.it tel: +39(0)554271234 > fax: +39(0)554271280 > > >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:06 EST