Hi Rees... Miltenyi Biotec makes a cup-style preseparation filter for their columns (#130-041-407) that looks quite similar to the Partec filters - however, they list the mesh size as 30 um, not 100. BD also makes cup filters for the Medimachine unit (called "filcons") that come in different mesh sizes and might be substitutable (100 um sterile are #340637 for 100 count, US$182). BD Falcon also makes sterile 40, 70 and 100 um cell filters that fit 50 ml tubes (100 um is #352360). We like to use these because they are relatively cheap and sterile - you just have to use them cautiously for tubes that are less than 50 mls. BD Falcon also makes their 12 x 75 mm FACS tubes with the filter cap, 40 um mesh I believe. As I recall, the Partec filters can be pulled apart, cleaned, their mesh replaced with Nytex 100 um screening and they can be reused. I don't know if the plastic can be autoclaved, however. Good luck, Bill Telford NCI-NIH At 05:08 PM 3/25/2002 +1100, Rees Beth wrote: >Hi Flowers > >Help!!! > >We've been using Partec's 100um filters to filter out bone particles and >cell clumps before staining bone marrow cells. > >I'm now told we're the only people in Australia who are using these, and our >supplier will not bother to import them after our latest order. What are >other people using, and where can I get it ? > >beth > >Beth Rees >Flow Cytometry Lab >Pathology Dept >Royal Hobart Hospital >Hobart, Tasmania >Australia > >ph. 03 6222 8913
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