Dear Flow folks: I am relatively new to this users group and new to flow so please bear with me. Our facility currently collects CD34+ stem cells for stem cell transplantation using apheresis. Our Apheresis collection center is interested in performing peripheral blood CD34 stem cell enumeration on patients prior to aphersis collection. The goal is to determine if a patient has an adequate number of CD34+ stem cells before performing the apheresis collection. They do not wish, necessarily, to collect the entire CD34 dose in one collection but, rather to ensure that there is adequate cd34+ stem cells such that 2-3 collections would yield an adequate dose for transplantation. Does anyone have any experience with this type of data. Are there some guidelines as to how many cd34+ stem cells /ul of peripheral blood are necessary to give an acceptable apheresis collection dosage? I am using an Epics XL and the Beckman/Coulter Stem-kit for CD34 enumerations. Thanks for any help. Patrick Patrick McMahon Avera Mckennan Hospital Sioux Falls, SD email Pat.McMahon@mckennan.org Fax: 605-322-7150
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