You put it in to prevent capping & endocytosis of Ab-bound cell surface protein. But leave it out if you are going to measure mitochondrial function or any ATP-requiring cellular process. If you have to do both kinds of measurements simultaneously, be sure to keep your cells on ice--that will also prevent capping & endocytosis. I always include it in FACS buffer--that it inhibits bacterial growth in the 1% serum or BSA is an added bonus. Beverly E. Barton, Ph.D. Assistant Professor Department of Surgery New Jersey Medical School Box 1709 185 S. Orange Ave. Newark, NJ 07101 phone 973-972-0662 fax 973-972-6803 e-mail bartonbe@umdnj.edu "Dr. Ashraf Abdelhafez" wrote: > Hi All: > > Does anybody have a conclusive answer to why azide is added to > staining buffer. I have found information that says that it functions > to inhibit microbial growth. I have found that it can be used to stop > reactions. I have found people recommending not to use in certain > experiment because it can alter protein function. And I have found > people saying that you do not need to put if you are going to analyze > the cells within hours. But I was not able to find information on that > exact reason for adding it to staining buffer. > > I would like to hear what you have. Only what you believe is a > conclusive answer is accepted. > > Thanks, > > Ashraf > > > ----------------------------------------------------------------------- > Do you Yahoo!? > New DSL Internet Access from SBC & Yahoo!
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