Dear Flowers,
Does anyone know of a protocol to stain specificaly a given plasmid
used in transfection, in order to be able to follow its uptake by cells
during different exposition times ?
I have been using DAPI a lot and know it's specific for double
stranded DNA (as is plasmid DNA) but was unable to find any staining
protocol out there, and I have to admit that I fear some stain leakage
and non specific signal if I use it !
If anyone know any staining procedure that can be used through flow
cytometry and will remain specific to plasmid DNA used, it would be much
appreciate.
Thank you for your help,
Best regards to you all,
Fred Grosjean
FSB - ISP
LBTC - EPFL
1015 Lausanne
Switzerland
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