The dissociation solution is expensive and you may obtain the same results with o.01M PBS with 60 mM EDTA. The EDTA is the active ingredient. It chelates divalent cations that aid in attachment allowing the cells to break loose without cellular damage. Tim Chance -----Original Message----- From: Dee Harrison-Findik [mailto:duygu.harrison-findik@imvs.sa.gov.au] Sent: Wednesday, March 13, 2002 6:30 PM To: cyto-inbox Subject: Macrophage harvesting, GIBCO buffer Dear Flowers Chinnaswamy has kindly forwarded the details of the cell dissociation buffer from GIBCO to me as another alternative to harvest macrophages. His message is below. Cheers Dee -----Original Message----- From: Chinnaswamy Jagannath [mailto:Chinnaswamy.Jagannath@uth.tmc.edu] Sent: Thursday, 14 March 2002 12:11 am To: cyto-inbox Subject: GIBCO buffer Ms. Dee: here are the specs for the GIBCO cell dissociation buffer; please forward to appropriate people... Cat NO: 13150-016 Component NO: 1096537 cell dis buf, enzyme free, HBSS based propritary formual for detaching cells from plastic surfacces;cells can thne be tested for flow, ligand binding, immunohstochemistry etc Make: GIBCO BRL I have tested such cells for mannose receptor and it works just fine; I strongly recommend it for macrophage studies. Dr.Jagannath
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