Dear Flowers Chinnaswamy has kindly forwarded the details of the cell dissociation buffer from GIBCO to me as another alternative to harvest macrophages. His message is below. Cheers Dee -----Original Message----- From: Chinnaswamy Jagannath [mailto:Chinnaswamy.Jagannath@uth.tmc.edu] Sent: Thursday, 14 March 2002 12:11 am To: cyto-inbox Subject: GIBCO buffer Ms. Dee: here are the specs for the GIBCO cell dissociation buffer; please forward to appropriate people... Cat NO: 13150-016 Component NO: 1096537 cell dis buf, enzyme free, HBSS based propritary formual for detaching cells from plastic surfacces;cells can thne be tested for flow, ligand binding, immunohstochemistry etc Make: GIBCO BRL I have tested such cells for mannose receptor and it works just fine; I strongly recommend it for macrophage studies. Dr.Jagannath
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