I stopped running isotype controls in any established situations where it could be shown that they were no different from the no-antibody control (do you also run these?) - and so seemed like a waste of money, sample and time - hardly no-brain reasons. Robin Barclay PhD Lead Scientist / Honorary Senior Lecturer SNBTS Academic Transfusion Medicine Group John Hughes Bennett Laboratory University of Edinburgh Dept of Oncology Western General Hospital Edinburgh ----- Original Message ----- From: "Julie L Wieseler Frank" <frankjw@psych.colorado.edu> To: cyto-inbox Sent: Tuesday, May 21, 2002 4:35 PM Subject: controls > > hello all! > > okay, so when i was trained to run flow experiments, i was STRONGLY > encouraged to use isotype controls for each label i used to evaluate non- > specific binding. is this considered a highly conservative approach? i am > working with somebody who shrugs off my need/want to run isotype controls, > and i feel baffled. > > i appreciate feedback -- this seems like a no-brainer, but it would seem i > am missing something! > > -- > Julie L. Wieseler-Frank, PhD > Research Associate > Department of Psychology > Campus Box 345 > University of Colorado at Boulder > Boulder CO 80309-0345 > tel: 303 735 2295 > fax: 303 492 2967 > frankjw@psych.colorado.edu > >
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