hello all! okay, so when i was trained to run flow experiments, i was STRONGLY encouraged to use isotype controls for each label i used to evaluate non- specific binding. is this considered a highly conservative approach? i am working with somebody who shrugs off my need/want to run isotype controls, and i feel baffled. i appreciate feedback -- this seems like a no-brainer, but it would seem i am missing something! -- Julie L. Wieseler-Frank, PhD Research Associate Department of Psychology Campus Box 345 University of Colorado at Boulder Boulder CO 80309-0345 tel: 303 735 2295 fax: 303 492 2967 frankjw@psych.colorado.edu
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