Hello Julie, There were some very good responses from various people last year on this subject -worth checking back on the archive. Arnold > hello all! > > okay, so when i was trained to run flow experiments, i was STRONGLY > encouraged to use isotype controls for each label i used to evaluate non- > specific binding. is this considered a highly conservative approach? i > am > working with somebody who shrugs off my need/want to run isotype controls, > and i feel baffled. > > i appreciate feedback -- this seems like a no-brainer, but it would seem i > am missing something! > > -- > Julie L. Wieseler-Frank, PhD > Research Associate > Department of Psychology > Campus Box 345 > University of Colorado at Boulder > Boulder CO 80309-0345 > tel: 303 735 2295 > fax: 303 492 2967 > frankjw@psych.colorado.edu > > > _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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