Dear all, I am always doing cell surface marker expression on hematopoietic cells from patients and healthy donors. I really enjoy the performance and more encouraging results. Now I start to touch intracellular antibodies such as MPO and lysozyme and hematopoietic cell lines. I found it is much more difficult to FACS analysis of these in-cell markers expression in the cell lines such as KG-1a. Does anybody has this experience and is so kind to give me any recommendations? Any concerns of this question should be greatly appreciated! I am looking forward to any response. Yalin
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