We have a FACSFlow system which is fantastic. We have not had a single problem with it. We received it with our MAS (96 well plate autosampler) which we are also very pleased with. BD may be selling the two units as a system which is why there may be a problem obtaining the FACSFlow separately. Paul Smith lab Moffitt Cancer Center Tampa, Florida 813-903-6871 > ---------- > From: Akos Szilvasi > Sent: Tuesday, July 16, 2002 8:54 AM > To: Cytometry Mailing List > Subject: Re: Instability with large sheath tank > > > We ordered a Calibur over a year ago WITH the FACSFlow included in the > quote. BD could not deliver the FACSFlow for a year. We were told by our > rep that it was not available in the US. That's the last time we heard > about it. > > Akos > > > > > > "Richard Konz" <RKonz@wyeth.com> > > 16-Jul-2002 07:54 AM > > To: Akos_Szilvasi@biogen.com, > cytometry@flowcyt.cyto.purdue.edu > cc: > Subject: Re: Instability with large sheath tank > > > > Akos and Gary: > > Actually, I have three of the FACSflow systems on order from BD > Biosciences. They are supposed to be available > for sale in the US in a month or so. They are available for the 'Calibur > or can be retrofitted for FACScans. > > Contact your BD rep. for details. > > -Rich > > > Richard F. Konz, Jr. > Scientist > Manager, Flow and Image Cytometry Facility > Wyeth > 200 Cambridge Park Drive > Cambridge, MA 02140 USA > Office: 617-665-5522 > Lab: 617-665-5551 > > >>> "Akos Szilvasi" <Akos_Szilvasi@biogen.com> 07/09/02 02:02PM >>> > Gary, sometimes the plastic tanks develop very small leaks that hard to > detect. Try a new one and see if it makes a difference. We have a BD > FACSFlow system that can use 20 liters tanks. Great addition to the > Calibur BUT you can not buy it. They do not sell it in the US. Try Cytek > (http://www.cytekdev.com). They have a large tank supply system for the > FACSCan/Calibur. > > Best regards, Akos > Biogen > > > > > > Gary.A.Ward@dartmouth.edu (Gary A. Ward) > 05-Jul-2002 10:35 AM > > > To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> > cc: > Subject: Instability with large sheath tank > > > > Hello Flow Network, > > > I'm looking for advice from anyone with experience using a FACScan or > FACSCalibur with a large sheath tank. We have been experiencing > instability > with our fluorescent signals vs time as well as an apparent decrease in > fluorescence intensity. I've checked for air leaks at the tank and > believe it > to be stable. I've also checked for air bubbles in the Pall filter and > tubing > and these seem to be clear. We routinely clean our system so I don't > believe it > to be dirty. The history of the laser indicates it to be stable. Any > other > suggestions? > > > Also what PSI air pressure are users running with the large tanks? > > > Thank you! > > > Gary A. Ward > > H C Englert Cell Analysis Laboratory > > Norris Cotton Cancer Center > > Lebanon, NH USA > > > > > > >
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