We currently do this experiment on both the FACStar plus and the FACS Vantage. The populations are usually very similar. The problem usually is associated with the staining. We have found that the cell nomber and concentration of dye are critical to generating good profiles. We stain mouse BM at 1 million per ml with 4ug/ml Hoechst for 60 min. When we sort on the Vantage at hagher rates, we concentrate the cells after staining to 3-5 million per ml. The profiles are identical. Charles A. Kuszynski, Ph.D. Assistant Professor/Director Cell Analysis Facility University of Nebraska Medical Center 986495 Nebraska Medical Center Omaha, NE 68198-6495 402 559-6267 voice 402 559-4077 fax ckuszyns@unmc.edu
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:25:55 EST