Joseph, Different devices may allocate different block sizes in their file allocation procedures for the same files. Also, forgive me for stating what may be obvious - but 256 channel data (8 bits) requires 1 byte (8 bits) per data point, 1024 channel data (10 bits) requires 2 bytes (16 bits) because most systems use bytes as the "smallest" unit. Similarly, the newer digital systems require 4 bytes (32 bits) for 18 bit, 20 bit, 24 bit, and 32 bit data. So, expect the binary part of a FCS file double from 256 to 1024 channel data, and for it double again if 32 bits are required. Since FCS files have a short header and variable TEXT info section, the initial TEXT part of the FCS file will typically vary in size from manufacturer to manufacturer, but remain about the same modest size no matter what the binary portion requires. Best regards, Joe Joseph Webster <J.Webster@centenary.usyd.edu.AU> on 03/19/2002 08:37:07 PM To: cyto-inbox cc: Subject: Re: 256 vs. 1024 channels Hmmmmmm Many thanks to all those who showed me the bullet hole in my shoe... A few days ago I wrote: > > .... but remember that a 1024-channel data file is four times > > bigger than the same data in 256 channels. After a prompt from Mario, I checked this morning and that statement is wrong! I remember a factor of four in the (good old?) days of HP computer and LYSYS II, but had never bothered to check that change in newer computers. Testing this morning, my 256-channel file was 72078 bytes while the same data (test pulses) in 1024-channel was 142078 bytes, or about double the file size. Just to confuse matters though, the Mac file system uses 130Kb and 195Kb respectively for the same two files on a 2Gb disk.... Now the accuracy of my memory is quite a different matter... ;~) Anyone still running a FACScan with LYSIS? Joseph.
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