Hi Jess, If you are using a cell line to monitor effects of a drug my question would be why do you need a normal population of cells? Even though your cell line is presumably transformed and may well be aneuploid, the control for your experiment would be a normal exponentially growing sample of the cell line. This will have a G1 peak, S phase and G2 - they may not be in the same place as 'normal' lymhocytes but you would be able to see your drug effects compared to your untreated control Derek. >I am attempting to do DNA analysis via propidium iodide staining on the flow >for the first time. My question is in regards to controls. The project we >are working on involves looking at a human breast tumorigenic cell line and >determining if various drug treatments have an effect on the cell cycle. >Since we are not a clinical lab, we do not have easy access to human >peripheral blood samples. Is there any way to obtain a normal population of >human cells to use as a control commercially or are there other controls >that can be used? > >thank you, > >Jess ************************************************************************ Derek Davies Voice: (44) 020 7269 3394 FACS Laboratory, FAX: (44) 020 7269 3100 London Research Institute, e_mail: derek.davies@cancer.org.uk Cancer Research UK mobile: 07790 604112 44 Linolns Inn Fields, London, UK. Web Page: http://sci.cancerresearchuk.org/axp/facs/davies/index.html In tenebris lux *************************************************************************
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