dna analysis controls

From: Jess Jankowsky (jankowsky.1@osu.edu)
Date: Thu Oct 24 2002 - 10:30:34 EST


I am attempting to do DNA analysis via propidium iodide staining on the flow
for the first time.  My question is in regards to controls.  The project we
are working on involves looking at a human breast tumorigenic cell line and
determining if various drug treatments have an effect on the cell cycle.
Since we are not a clinical lab, we do not have easy access to human
peripheral blood samples.  Is there any way to obtain a normal population of
human cells to use as a control commercially or are there other controls
that can be used?

thank you,

Jess



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