Mast cells and basic orange 21

From: Amy Raber (araber@athersys.com)
Date: Fri Oct 25 2002 - 13:57:41 EST 

Dear Flow users,

I am interested in stimulating a mast cell line and using flow cytometry
to screen for degranulation.  I came across mention of a dye-basic
orange 21 that may be appropriate for this assay.  I was hoping someone
would know where I can purchase this dye?  Is there a protocal somewhere
for staining?  Or is there a better method to measure degranulation?
(we are looking into annexin staining for degranulation too)  We only
have access to 488nm laser.


Thanks so much for any help you can send my way!!

Amy

Amy Raber
Athersys Inc.
Cleveland, OH

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