Hi All, Matthias has kindly pointed out that it was important to mention which version of the Coulter software I was using - it's Expo32 v1.2 Analysis. Mnay thanks, Elaine Kennedy Organon Laboratories Ltd. Scotland Hi All, I've been trying to figure out the meaning of the stats on the cytometer we use and find them very confusing, I was hoping some of you experts may be able to help me out a bit with them! Using the software with the machine I am able to get the following stats calculated automatically:- - Chi2 - K-S Mode probability - K-S Mode D value I'm also not sure what the overlay plots should look like in K-S mode i.e. what should I expect from 2 populations which are different? One of the problems I have is that we are looking at 2 different populations of cells and even when there appears to be very little difference between them the K-S probability is still coming out very low (it seems to always give a value of <0.000001 no matter how similar/different the populations are). To help illustrate these problem I attach a word file containing 2 separate analyses, one in which from looking at the basic overlay plots you would expect there to be a significance in the stats and the other in which the populations appear to be too similar for the stats to give you significance. I would also welcome any comments you may have generally on the use of stats with flow results. Thanks for your help, Elaine Kennedy Organon Laboratories Scotland <<not significant.doc>> <<significant.doc>> -------------------------------------------------------------------- This message, including attached files, may contain confidential information and is intended only for the use by the individual and/or the entity to which it is addressed. Any unauthorized use, dissemination of, or copying of the information contained herein is not allowed and may lead to irreparable harm and damage for which you may be held liable. If you receive this message in error or if it is intended for someone else please notify the sender by returning this e-mail immediately and delete the message. -------------------------------------------------------------------- -------------------------------------------------------------------- This message, including attached files, may contain confidential information and is intended only for the use by the individual and/or the entity to which it is addressed. Any unauthorized use, dissemination of, or copying of the information contained herein is not allowed and may lead to irreparable harm and damage for which you may be held liable. If you receive this message in error or if it is intended for someone else please notify the sender by returning this e-mail immediately and delete the message. --------------------------------------------------------------------
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