Hello, Does anyone have any experience with serum starving mammalian tissue culture cells to synchronize cell cycles? I am interested in how long to serum starve, what percentage of FBS to use, and the approximate time points to look at after release from the block. I would greatly appreciate any help. Many thanks, Greg -- ******************** Greg Crawford, Ph.D. National Human Genome Research Institute National Institute of Health Genome Technology Branch 50 South Drive, Room 5309 Bethesda, MD 20892 tel: 301-594-9203 fax: 301-480-9667
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