Here is a question for all of you mouse immunologists out there. The immunophenotype of human memory B cells is CD20+, CD38-, CD44+ and CD86+. In mice you need to use CD138 instead of CD38 to exclude plasma cells. If you look at CD19 or B220+, CD138-, CD44+ and CD86+ cells in mouse spleen post immunization do you detect memory B cells? Are plasma cells CD44+? Also- does MUM-1 expression mean plasma cell differentiation or can this be expressed in memory B cells or pre-plasma cells? Maryalice Stetler-Stevenson, M.D., Ph.D. Chief, Flow Cytometry Unit Laboratory of Pathology, NCI, NIH Sometimes you're the windshield, sometimes you're the bug.
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