Hi Nadeem,
We routinely do CSF testing for tumor. The volume we receive is usually
3-5cc. I don't know if we have ever received a normal as most of the
patients have AIDS or LYG and the MD is looking to see if a secondary
malignancy has developed. We spin the specimen down and decant all but about
50ul. I use 6.5ul times 2 to look at viability and do a cell count with
crystal violet. If any RBC are seen in the trypan blue tube, it is noted and
put in the report.
The most cells I have counted was 300,000 which means that you have to have
a fair idea of what to look for first.
Hope this helps,
Paula
-----Original Message-----
From: Nadeem Sheikh [mailto:nadeem@bart.rprc.washington.edu]
Sent: Thursday, January 31, 2002 1:23 PM
To: cyto-inbox
Subject: CSF Immunophenotyping
Can anyone point me in the right direction for immunophenotyping cells from
cerebrospinal fluid. Specific questions I have are:
1. What kind of volume is usually taken
2. What are the normal population of cells - phenotype, nos. ?
3. Any safeguards/controls for blood contamination ?
I should point out that we are looking to establish techniques to look at
cellular infiltrates (if we can isolate any) in CSF of macaques infected
with SIV/SHIV that subsequently develop NeuroAIDS. Any advice, pointers to
papers would be most welcome human or macaque work.
With regards,
Nadeem
Nadeem Sheikh, PhD
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RPRC
University of Washington
Box 357331
Seattle, WA 98195
Voice : +1 206 221 2356
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e-mail : nadeem@bart.rprc.washington.edu
nadeem@u.washington.edu
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