You might try fluorescein diacetate (FDA) from molecular probes. It stains the cells green if they are alive. Good Luck Michal Abel wrote: > I've got a question for the list regarding staining for viability. > I am working with freesed/defrost lymphocytes. One of the drawbacks > of this procedure is the number of death cells, which make higher the > background noise. I wonder what kind of marker should I use for > gating only the alive lymphocytes. Logically, I thought, the best > would be necrosis marker like PI. However, I am not sure if I also > need to eliminate the early apoptotic cells or not ? > I would be grateful if someone could help me resolving this problem. > > Thanks, > > -- > Michal Abel, MD, PhD > Laboratory of Clinical Immunology > INSERM U25 > Necker Hospital, 161 Rue de Sèvres. Paris > tel: 33 1 44 49 52 23 > fax: 33 1 44 49 53 74
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