There are multiple published examples of SYTO 13 (green fluorescent) and TO-PRO-3 (red fluorescent) for bacterial counting and viability http://www.probes.com/servlets/bib?item=7575 http://www.probes.com/servlets/bib?item=3605 James Hall wrote: > Glad to have something to contribute at last........we regularly count > bacteria of 1um size down to 1000 cells/ml, and we use the viability kits > to a similar sensitivity. If you want to just enumerate we have found > SYTO-13 to be excellent and then count on side scatter and FL1, we use a > FACScan. We also use SYTO 13 and a cell impermeable dye like TOPRO (the > dimer, I forget the number) and count on FL1 and FL3 to distinguish > viability. An authority on this is Andy Beavis, who was at Princeton but > has now moved on to a better place. Are you out there Andy.....? > > James > > Dr. James Hall > Dept. of Geosciences > Princeton University > Princeton, NJ,08544 > Phone: (w) 609-258-2597 > (h) 609 919 0292 > e-mail hall@princeton.edu
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:16 EST