I've been digesting previous emails re: flow rate and coincident events. The basic advice is: go slower, reduce the chance of coincident events crossing the laser. In fact, I was taught from wayyyy back: keep the flow rate between under 1000 (optimum) for most cell types. For DNA work, around 100. But clearly the rate displayed (in the "counters" window ) is a reflection of the threshold. So when you adjust the flow rate, do you decrease the threshold to get a more accurate picture of the events flowing by, adjust the flow rate, then threshold out the events you don't want during collection? (When you run lysed whole blood, or harvest from cultures, it really always practical to adjust the cell number in the tubes). So when one is advised to keep a flow rate "between XXX and YYY"- is this assuming a threshold value of "ZZZ"?? Or after you establish your ideal settings? thanks for the input- -- Bunny Cotleur, M.S. Sr. Technologist Cleveland Clinic Foundation Neurosciences NC30 9500 Euclid Avenue Cleveland, OH 44195 cotleur@ccf.org (216) 444-1164 fax (216) 444-7197
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