Here's a good reference for monocyte isolation. Just go to this web site for a summary,
as well as a link to the article secribing the protocol in detail.
http://memorias.ioc.fiocruz.br/952/3879.html
Good luck
kb
-------------
Keith Bahjat, Ph.D.
Scientist, Cancer Vaccines
Cerus Corporation
Concord, California
keith_bahjat@cerus.com
On 7/28/2002 1:46 PM, Wolber, Fran <F.M.Wolber@massey.ac.nz> wrote:
> Clean Clean DocumentEmail MicrosoftInternetExplorer4
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>
>Calin
>
>Try looking up a paper from around 1990-ish, in J Imm Methods, by
>Elizabeth Denholm, on isolating human monos using a modified Percoll
>gradient.
>
>
>Fran Wolber
>Institute of Food, Nutrition and Human Health
>Massey University
>
>-----Original Message-----
>From: Calin Tatu [mailto:cta@med.unc.edu]
>Sent: Friday, 26 July 2002 4:22 a.m.
>To: Cytometry Mailing List
>Subject: monocyte isolation
>
>
>Dear Flowers,
>
>
>
>Does anyone have a reliable protocol for density gradient separation
>of human monocytes from whole peripheral blood? We have tried several
>Fycoll densities and osmolarities but none seems to work. We have
>been using magnetic isolation on MACS but we are looking for a more
>expedient and cheaper way to get pure (i.e. >90%) monocytes.
>
> Any reply would be greatly appreciated.
>
>
>
>Thank you,
>
>
>
>Calin Tatu.
--
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