Hi Ruth, these suggestions depend on how enriched your cell population is, but you could use anti-CD11b or anti-CD16. CD11b is also expressed on monocytes, but only a small population of circulating monocytes express Fc gamma RIII (CD16). I know you can get anti-CD11b in RPE format because I have used some from Serotec in the past, and gated on a region positive for granulocytes in an enriched suspension. Pretty sure the same would work for 3G8 clone of anti-CD16. Craig On Fri, 8 Feb 2002 Ruth_Wilkins@hc-sc.gc.ca wrote: > Does anyone know of a human antibody for neutrophils that is available in any > colour besides FITC? I am trying to measure Annexin labelling in neutrophils > and am having problems with Annexin-PE but Annexin-FITC works well. ************************************************************************ Derek Davies Voice: (44) 020 7269 3394 FACS Laboratory, FAX: (44) 020 7269 3100 Cancer Research UK, e_mail: derek.davies@cancer.org.uk London, UK mobile: 07790 604112 Web Page: http://sci.cancerresearchuk.org/axp/facs/davies/index.html In tenebris lux *************************************************************************
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