Out of interest - what "anti-neutrophil" antibody are you using? I find CD16 OK for freshly isolated neutrophils - but not so good if the blood is a little older (a few hours to overnight). I don't know of any marker that is neutrophil-specific, not shared by monocytes (whereas monocytes have CD14 not found on neutrophils). I'm just looking for something practical to follow neutrophil maturation in vitro from CD34+ cells cultured with various cytokine cocktails - and would appreciate any advice. Robin Barclay _____________________________ Dr G Robin Barclay PhD Lead Scientist / Honorary Senior Lecturer SNBTS Academic Transfusion Medicine Group John Hughes Bennett Laboratory Western General Hospital, Edinburgh, UK EH4 2XU Tel: ++44 (0) 131 537 3150 Robin.Barclay@ed.ac.uk ----- Original Message ----- From: <Ruth_Wilkins@hc-sc.gc.ca> To: cyto-inbox Sent: 08 February 2002 21:29 Subject: neutrophils > > > > Does anyone know of a human antibody for neutrophils that is available in any > colour besides FITC? I am trying to measure Annexin labelling in neutrophils > and am having problems with Annexin-PE but Annexin-FITC works well. > > Thanks, > > Ruth Wilkins, Ph.D. > Consumer and Clinical Radiation Protection Bureau > Health Canada > 775 Brookfield Road, PL 6303B > Ottawa, ON K1A 1C1 > > (613) 941-7263 > FAX (613) 941-1734 > >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:25:59 EST