Patrizia I have had similar problems with Calphostin C in studying the role of PKC on differentiation of haematopoietic cells. In the course of these experiments I came across a report which claimed that the action of this inhibitor was light dependent making this an uncontrolled variable in most peoples experiments. I was unable to find confirmation of this, but I did get much more reproducible results with an alternative inhibitor, GF109203X (1-2 uM). R. F. Bruns, F. D. Miller, R. L. Merriman, J. J. Howbert, W. F. Heath, E. Kobayashi, I. Takahashi, T. Tamaoki, and H. Nakano. Inhibition of protein kinase C by calphostin C is light-dependent. Biochem.Biophys.Res.Commun. 176 (1):288-293, 1991. Dr Richard Darley Ph.D Department of Haematology A7 University of Wales College of Medicine Cardiff, CF14 4XN U.K. TEL +44 (0)29 2074 3485 or +44 (0)29 2074 2375 Fax +44 (0)29 2074 4655 e-mail: darley@cf.ac.uk
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