Stange request but I would suggest either sonicate them or lyse them with detergent. 0.5% triton X would probably do the job. Simon Monard Trudeau Institutue NY >>> Michael Corey <coreym@targen.com> 12/23/02 15:34 PM >>> Hi Folks, My first posting. I'm pretty new to FACS (most of my experience is in enzyme assays and molecular biology). What I'm trying to do right now is smash up some PBMCs to generate debris for use as a standard to characterize naturally occurring debris from expansion cultures. I tried freeze-thaw but got only aggregated material--I need a method that solubilizes everything, so that I can FACS the debris from a defined number of cells. What do people think? Thanks much and happy holidays, Michael -- Michael J. Corey, Ph.D. Associate Director, Assay Development CellExSys 1100 Olive Way, Suite 100 Seattle, WA 98101 USA Tel.: 206-521-4846 Fax: 206-521-4841 Email: coreym@targen.com
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