Dear José, I have not worked with CD27 a great lot, however, I can recommend a good source of references on naive vs. memory studies: Dr. Roederer has a paper (Nature Medicine 7, 245 - 248 (2001). )on multicolor flow analysis of memory vs. naive in Nat Med, see http://www.nature.com/cgi-taf/DynaPage.taf?file=/nm/journal/v7/n2/full/nm020 1_245.html as well as the references in that paper to see a discussion on all the memory vs. naive markers. I use that paper very often especially whenever I need to prove someone wrong about doing 2 color memory vs. naive studies. Also I wonder - for anyone else who reads this thread - could you comment on other phenotypes that are missing after thawing (or ways to perserve them?) I am definitely noticing lack of CD56 and reduced CD19, its good to know that CD62L is also reduced (though I will have to try it with my frozen cells to verify it). Maciej Simm Lab Dude Cornell University Medical Center Peds Hem/Onc Cunningham-Rundles Lab 212 746 3428 voice 212 746 8573 fax ----- Original Hi everyone, I am working with frozen PBMCs and using CD27 in combination with CD45RA to define naive cells (RA+27+). My idea is that CD27 can be used instead of CD62L to define these cells, because expression of CD62L is lost in frozen cells. I assume this is based in the fact that in certain subsets these two molecules are coexpressed in the same cells, and in fact I have found that this is the case in the CD8 subset (all CD62L+ cells are CD27+). My question is: is this just a coincidence or is that CD27 function is related to the naive phenotype? Many thanks José Miguel Benito
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