Dear Jose, >From our experience I can inform you that RA+CD27+ are always CD62L+ therefore CD27 could as well be used in stead of CD62L. On the other hand after antigen encounter the cells will also express CD45RO. In this phenotype there will be a difference in the expression of CD27 en CD62L since CD62L will be down regulated after homing where as CD27 stays unchanged, for that reason CD27 is our favourite to use as marker. There are several publications on the function of CD27, on naïve cells and it seems to be involved in memory formation. Perhaps less appropriate but I will use this mail to inform you that we have made a superior monoclonal antibody against CD27 (CLB-CD27/1, 9F4), specially the CD27-FITC conjugate gives a much brighter staining then any other clone tested in our lab. To prove this I am offering free samples to everyone who is interested to test this. Best regards, John John Voorn Sanquin Reagents Product manager Plesmanlaan 125 j_voorn@clb.nl <mailto:j_voorn@clb.nl> 1066CX AMSTERDAM the Netherlands tel +31-(0)20 5123246 fax +31-(0)20 5123570 web site www.clb.nl <www.clb.nl> -----Original Message----- From: Jose Benito [SMTP:jbenito1@hotmail.com] Sent: Tuesday, June 18, 2002 3:17 PM To: Cytometry Mailing List Subject: CD27 and CD62L Hi everyone, I am working with frozen PBMCs and using CD27 in combination with CD45RA to define naive cells (RA+27+). My idea is that CD27 can be used instead of CD62L to define these cells, because expression of CD62L is lost in frozen cells. I assume this is based in the fact that in certain subsets these two molecules are coexpressed in the same cells, and in fact I have found that this is the case in the CD8 subset (all CD62L+ cells are CD27+). My question is: is this just a coincidence or is that CD27 function is related to the naive phenotype? Many thanks José Miguel Benito Hospital Carlos III Madrid _____ Chat with friends online, try MSN Messenger: Click Here <http://g.msn.com/1HM500901/154>
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:13 EST