use of GFP and CFP reporters with a VantageSE

From: Andrew Herman (A.Herman@bristol.ac.uk)
Date: Wed Oct 23 2002 - 06:11:37 EST


Dear Flow-ers,
  I have a colleague who wishes to analyze and sort cells that bear GFP
and CFP (cyan fluor. protein) reporter constructs.
Has anyone had experience doing this type of analysis with a Vantage SE
and an Enterprise II laser (6 color config). I realize that using a 488
nm line is suboptimal for CFP, but is it feasible?

Any info would be very much appreciated.

Thanks,

----------------------
Andrew Herman
Department of Pathology & Microbiology
University of Bristol
University Walk
Bristol  BS8 1TD
Tel. +44 117 928 7511
Fax. +44 117 928 7896
A.Herman@bristol.ac.uk



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