Interesting question. The chemiluminescence peak of luciferin is at about 560 nm, which is a bit shorter wavelength than phycoerythrin emssion. Of course, it does not need any excitation to get the emission. But then, I don't know if the rate of emission will be fast enough to get enough photons during the transit time that you will be able to detect the signal. But then S/N should be great because all photons should be "real." I don't know what technical difficulties there may be doing sorting with the laser turned off but hope it works. Nan Jiang wrote: >Dear all: > >I am wondering if anybody ever sorted cells infected with luciferase >plasmid. Or is there a particular wavelengh we can use to detect >luciferase activity in cells and sort them based on that. > >Thanks in advance. > >Nan Jiang > >Department of Internal Medicine >Cardiology Division >UT Southwestern Medical center at Dallas >6000 Harry Hines Blvd. >Dallas, TX 75390-8573 >(214) 648-1175 >(214) 648-1181 > > >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:31 EST