Apoptotic assay for fibroblasts

From: Gerard Bowe (gerard.bowe@jrc.it)
Date: Tue Feb 12 2002 - 05:29:02 EST 

Hello everyone,

We are looking for a reliable apoptotic assay for Balb/c 3T3
(fibroblasts). We have tried the Annexin V FITC/PI with little success.
After inducing apoptosis by serum removal we get PI +ve, but FITC -ve.
We've tried various methods of inducing apoposis, but got little or no
FITC +ve. We detach the cells using trypsin.

Any suggestions/ help would be appreciated.


Thanking you,
Ger

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