Re: Intracellular pH

From: Howard Shapiro (hms@shapirolab.com)
Date: Sun Sep 29 2002 - 15:58:33 EST


Rob McCord wrote-

>I'm looking for a marker to measure intracellular pH.   Practical Flow
>Cytometry (3rd)
>mentions both an unnamed UV excited dye used, and BCECF-AM for 488 nm
>excitation.
>Are these still the most widely used markers?

The UV-excited dye was never that widely used; BCECF-AM is very widely
used, and comes from Molecular Probes, as do a variety of other
pH-sensitive dyes in the SNARF and SNAFL families. Look at the Molecular
Probes website for references.

-Howard



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