Monocytes and Macs

From: Annette Byrne (AByrne@pcyc.com)
Date: Tue Nov 26 2002 - 17:12:45 EST


 Hi Flowers

 I'm looking for some advice on the best way of separating out	the monocyte
population from peripheral blood and also  how does one go about activating
them to become terminal macrophages ?.

 Also for those of you in the know, is this the best system to look at human
Macs. To date I've been using PMA activated THP-1 cells but am trying to get
a more " physiological" way of doing things

 many thanks as always

 Annette

Annette Byrne PhD
Scientist
Pharmacyclics
995 E Arques Ave
Sunnyvale
CA 94085-4521
USA

Tel: 001-408-3283640
Fax: 001-408-3283689
e mail : AByrne@pcyc.com
www.pharmacyclics.com



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