Justin Fishbaugh wrote- >We have an investigator that needs the ability to fix human blood and stain >later for surface and possibly intracellular antigens. A search of Medline >came up with Jani et al, "Affordable CD4+ T cell counts by flow cytometry >II. The use of fixed whole blood in resource-poor settings," Journal of >Immunological Methods, Volume 257, Issues 1-2, Pages 145-154. This article >talks about using a fixative called TransFix made by UK NEQAS before >staining. A search of the UK NEQAS website did not find anything >referencing TransFix. Does anyone have experience with this stuff or have >some other magic potion that would do the trick? Thanks. David Barnett <d.barnett@sheffield.ac.uk> developed TransFix; if there isn't anything about it on the NEQAS site, he should be able to provide details. -Howard
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:30 EST