I had looked for a commercial mAb to this marker a while back and came up empty. The only Ab I'm aware of is 97A6. Do you or anyone else know of a source of this? Wayne Shreffler Mount Sinai School of Medicine On 10/7/02 4:01 PM, "Repetti, Charles" <crepetti@aml.com> wrote: > > Hi; > Consider basophils. > > FSC,SSC similar to Lymphs but CD45 slightly weaker > 13+33+ > 11b+15- > > 34-DR- > > Try CD203c-PE for baso. > > Best wishes > Charles > > > > -----Original Message----- > From: Pal Jakso [mailto:Pal.Jakso@aok.pte.hu] > Sent: Monday, October 07, 2002 2:46 AM > To: cyto-inbox > Subject: Re: Myeloid populations in myeloproliferative disorders > > > > Dear Emanuel, > > You are not alone. I have seen this population of cells many times not > only in chronic phase of CML but in MDS or CMPD too always as a > distinct population (1-8% of the white blood cells). These cells are > myeloid cells which have a particular position on the CD45/SSC plot > where normally myeloid cells are not presented. The phenotype you > described is very consistently myeloid always CD13 and CD33 positive, > lacking lymphoid markers and never shows CD34 positivity. Interestingly > these cells are in the lymphocyte region on the FSC/SSC plot. After > several discussions with my pathologist colleague we concluded that > these cells may be some kind of micromyeloblasts. > I'm also interested in any knowledge or idea of this great flow > community about this myeloid population. Has the presence of these > cells any diagnostic or prognostic role in the above mentioned myeloid > diseases ? > Please let me know if you'll get responses exluding the mine. > > Best regards, > Pal Jakso > > University of Pecs > Faculty of Medicine > Dept. of Pathology > 7643, Hungary > 12. Szigeti str. > > > On Friday, Oct 4, 2002, at 02:52 Europe/Budapest, Emanuel Raniolo wrote: > >> >> Hi Everyone >> >> Can anyone fill me in on the identity of a cell population that I have >> seen >> recently in 2 patients with myeloproliferative disorders. >> The first patient was a chronic phase CML, the bone marrow of which >> displayed a CD45+dim (slightly less than lymphocytes), intermediate >> side >> scatter (less than monocytes) population on CD45/Log SS analysis. This >> population represented approximately 3% of all histogram events. The >> phenotype was CD13+ CD33+ CD11b+ CD15- CD14- CD34- CD117-. >> The second patient (primary thrombocythemia) bone marrow displayed a >> similarly positioned numerically smaller population comprising 1% of >> all >> histogram events. This patient was not extensively phenotyped, but >> again >> this population was CD13+ CD34-. >> >> Has anyone seen these populations previously in their analysis. Do they >> represent a mature or immature myeloid population? >> >> Your responses would be appreciated. Thankyou. >> >> Emanuel Raniolo >> Cell Analysis and Cyopreservation Laboratory >> Department of Haematology, IMVS Laboratories >> The Queen Elizabeth Hospital Division >> Woodville, South Australia. >> >> >>
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