Dear group, an investigator would like us to perform a Dendritic cells study in our flow lab. We are primarily a clinical flow lab and not very familiar with dendritic cells. Although he gave us several reference papers, we are still unsure about the panel of antibodies (?CD40, ?CD80, ?CD83, ?CD123), the procedure, interpretation and the pitfalls associated with most flow "studies". If anybody has experience with flow cytometric studies on dendritic cells, we would certainly appreciate your help and input. Thank you - Andrea
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