I am currently reviewing several lab protocols (for myself) and many labs include b-marcaptoethanol in their flavor of "complete" media. Searching the cytometry site and various 'net search engines bruoght me no closer to "WHY?" I am not familiar with media containing this supplement. I have found references to including it in basic cell culture, stimulation assays, freezing media, flow staining, etc so I can't even tie it to a particular technique. Can anyone enlighten me? Thanks- -- Bunny Cotleur, M.S. Sr. Technologist Cleveland Clinic Foundation Neurosciences NC30 9500 Euclid Avenue Cleveland, OH 44195 cotleur@ccf.org (216) 444-1164 fax (216) 444-7197
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