Hi Bunny,
To my knowledge 2-mercaptoethanol as a reducing agent can break down many of
the toxic metabolites produced by cells in culture thus improving the
environment around the cells. It is used more with the culture of mouse
cells than others. In my earlier days (too long ago!!) it was the magic
ingredient that made mouse cytotoxic T cells grow better. It'd be
interesting to know what others think. I hope I've helped.
All the best,
Sam
**********************
Dr Sam Hou
Lung Immunology Group
Edward Jenner Institute for Vaccine Research
Compton
Newbury
RG20 7NN
web site: www.jenner.ac.uk
Ph: 01635-577-924
Fax: 01635-577-901
-----Original Message-----
From: b cotleur [mailto:bunny@cotleur.com]
Sent: 17 April 2002 18:03
To: cyto-inbox
Subject: b-mercaptoethanol in media
I am currently reviewing several lab protocols (for myself) and many
labs include b-marcaptoethanol in their flavor of "complete" media.
Searching the cytometry site and various 'net search engines bruoght me
no closer to "WHY?"
I am not familiar with media containing this supplement. I have found
references to including it in basic cell culture, stimulation assays,
freezing media, flow staining, etc so I can't even tie it to a
particular technique.
Can anyone enlighten me?
Thanks-
--
Bunny Cotleur, M.S.
Sr. Technologist
Cleveland Clinic Foundation
Neurosciences NC30
9500 Euclid Avenue
Cleveland, OH 44195
cotleur@ccf.org
(216) 444-1164
fax (216) 444-7197
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