Re: [cell synchrony technique]

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I am not sure about lymphoblasts, but for many immortalized cell lines
(and some primary cell lines), dropping the culture temperature to 28C is
a fairly gentle (and easily reversable) way to push most of the cells into
G0-G1 (a small percentage will remain in G2-M).  The synchronization is
complete within 48-72 hours.

Good luck.

Shari Brezinsky
Biogen





Gregory Crawford <crawford@nhgri.nih.gov>
04/17/2002 03:14 PM


        To:     Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
        cc:
        Subject:        [cell synchrony technique]




Hello,

I am interested in synchronizing K562 lymphoblast cells and was
interested in the most physiologically normal and effective method
for achieving this.  Does anyone have any experience with this?

Thanks,
Greg
--
********************
Greg Crawford, Ph.D.
National Human Genome Research Institute
National Institute of Health
Genome Technology Branch
50 South Drive, Room 5309
Bethesda, MD 20892

tel: 301-594-9203
fax: 301-480-9667

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