It turns out our RBC problem (few cells apparent by FACS compared with control tubes which contained no anti glycophorinA) was caused by agglutination. If anyone working in this area [titrating out a conjugated (ours has phycoerythrin) IgG anti glycophorinA antibody with the result that FACS analysis is possible, i.e., sufficient fluorescence remains] has any suggestions/advice which would allow us to use the antibody we already have, we would appreciate hearing from you. Thanks. Ray Hester rhester@jaguar1.usouthal.edu
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