What you are seeing is activation-induced cell death-likely apoptosis, characterised by the hallmark sactter changes that you describe-reduced FSC with increased SSC. This is a normal phenomenum observed associated with strong polyclonal T cell activators such as PMA/iono Gill Webster PhD Head of Flow Cytometry Genesis Research and Development PO Box 50 Auckland email: g.webster@genesis.co.nz direct dial 00 61 9 3735600 fax: 00 61 9 373 2189 -----Original Message----- From: Arumugham Raghunathan [mailto:ragoor@molecularstaging.com] Sent: Tuesday, May 07, 2002 8:56 AM To: cyto-inbox Subject: Lymphocyte activation Hi all, A question about activation of lymphs. When I activated lymphocytes using PMA and ionomycin, I see a population of cells with reduced side scatter properties apart from the regular lymphocytes. It looks like there are two populations (parallel shift) based on SSC. The shift is towards the left, and the number of cells shifting increases with activation-time. Has anyone observed this? What is it due to? Do the cells (lymphs) shrink upon activation? thanks, Ragoo *********************************************************** Arumugham Raghunathan, Ph.D Flow Cytometry Group Cellular Analysis Section Molecular Staging Inc., 300 George Street, Suite 701 New Haven, CT 06510 Tel# 203-772-5074 Fax# 203-776-5278 *************************************************************
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