I have never seen any change in FSC/SSC in red cells stained with anti-GpA. Is there any possibility that your cells are undergoing lysis? Robin Barclay SNBTS Academic Transfusion Medicine Group John Hughes Bennett Laboratory Edinburgh Tel: +44 (0)131 537 3150 (internal 33150) ----- Original Message ----- From: "ray hester" <rhester@jaguar1.usouthal.edu> To: cyto-inbox Sent: Monday, May 06, 2002 9:15 PM Subject: RBC and anti glycophorinA > > Greetings, > > Can anyone tell us if (IgG) anti glycophorin A antibody will cause > substantial changes in light scatter (particularly forward scatter) of > (human) RBC? We seem to see decreased forward scatter to the point of many > of the RBC almost being off scale to the left, and increasing the FSC gain > doesn't really help to bring these back on scale. This was just observed > today and the investigators will have to go back and look at the cells > microscopically after staining but I thought I would ask the group. > > Should they leave out added Ca and Mg in their binding and wash buffer, > i.e., are these (Ca/Mg)more of a problem with antibody staining of RBC than > lymphocytes? > > Thanks for any information. > > Ray Hester > College of Medicine > Univ. of South Alabama > rhester@jaguar1.usouthal.edu >
This archive was generated by hypermail 2b29 : Sun Jan 05 2003 - 19:26:09 EST